Transformation Of Arabidopsis Thaliana For Tandem Affinity Purification Of Chloroplast Proteins

Matrix attachment region-binding filament-like protein 1 (MFP1) is part of a chloroplast protein complex that interacts with the thylakoid membrane and chloroplast DNA. The proteins in this complex need to be identified in order to determine the complex’s function. Mechanisms such as tandem affinity purification can be used to identify the proteins that interact with MFP1. The purpose of this project is to use Agrobacterium tumefaciens to transform an MFP1-TAP expression construct into MFP1-knockout mutant Arabidopsis thaliana plants via floral dipping. The genotype of the mutants was confirmed via polymerase chain reactions (PCR). The success of the transformation was also confirmed via PCR. The goal of this project is to generate MFP1-TAP plants to purify protein complexes and identify the proteins that interact with MFP1