The southeastern United States, including Great Smoky Mountains National Park (GRSM), experience high ozone concentrations. These concentrations are high enough to cause visible injury on a wide variety of plants in GRSM. One plant that is particularly sensitive to the impacts of ozone is the perennial herb tall milkweed (Asclepias exaltata). This species may be a bioindicator for ozone at mid- to high elevations in the Park. However, little is known concerning the seasonal progression of injury on this species, nor the threshold levels of ozone necessary to elicit a response, both of which are necessary to better characterize this species for use as a bioindicator.